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byu researchers create tiny nano-device in newest gene therapy advance: nanoinjector is used to transfer genes and dna to new cellsthe ability to transfer a gene or dna sequence from one animal into the genome of another plays a critical role in the medical research of diseases such as cancer alzheimer039s and diabetesbut the traditional method of transferring genetic material into a new cell microinjection has a serious downside this method uses a hollow needle to pump a dna-filled liquid into an egg cell nucleus but that extra fluid causes the cell to swell and die 40 percent of the timenow a multidisciplinary team of brigham young university scientists has developed a way to significantly reduce cell death when introducing dna into egg cells the researchers have created a microscopic lance that delivers dna to the cells through electrical forcesquotbecause dna is naturally negatively charged it is attracted to the outside of the lance using positive voltagequot said brian jensen byu professor of mechanical engineering quotonce we insert the lance into a cell we simply reverse the polarity of the electrical force and the lance releases the dnaquotbecause the lance is 10 times smaller and no extra fluid is used the cells undergo significantly less stress compared to microinjection and thus have a higher survival rate the researchers describe their quotmetamorphic nanoinjectionquot process in an article published today by review of scientific instrumentscurrently the byu researchers which include microbiology professor sandra burnett and mechanical engineering professor larry howell are using the technique to inject dna into mouse zygotes single-cell embryos consisting of a fertilized eggquotthe microinjection technology hasn039t really changed over the last 40-50 years since it was inventedquot burnett said quotnot having to force liquid into the nuclei by shifting to a lance is a huge advantage it not only increases the survival rate but it also causes less damage for future developmentquotin research published in transgenic research the team found that 776 of nanoinjected mouse zygotes proceeded to the two-cell stage of development as compared to 547 for microinjected zygotesa major reason for creating transgenic animals is to research genetic or infectious diseases by modifying the genes of a mouse to carry a human disease researchers can generate data with insights into future treatments and therapies for those illnessesone of the byu team039s most significant findings is that it039s possible to use the electrical forces to get dna into the nucleus of a cell without aiming the lance into the pronucleus the cellular structure containing the cell039s dna this may mean that injections can be performed in animals with cloudy or opaque embryosquotsuch animals including many interesting larger ones like pigs would be attractive for a variety of transgenic technologiesquot jensen said quotwe believe nanoinjection may open new fields of discovery in these animalsquotjensen said more efficient injections should also reduce the cost to create transgenic animals jensen039s research is funded in part by the 400000 awarded to him in 2011 through a national science foundation career awardquentin aten a former phd student at byu now with nexus spine llc served as the lead author on the research published in review of scientific instrumentshttp:newsbyueduarchive14-may-nanoinjectoraspx

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